Results
| PMID | 25537670 |
| Gene Name | KRAS |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 31 |
| Population details | 31 ( 16 endometriosis, 15 controls ) |
| Sex | Female |
| Associated genes | K-Ras 4A, K-Ras 4B |
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Arch Gynecol Obstet. 2015 Jul;292(1):225-9. doi: 10.1007/s00404-014-3596-7. Epub Shahrabi-Farahani, Maryam| Shahbazi, Shirin| Mahdian, Reza| Amini-Moghaddam, Soheila Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. AIMS: K-Ras transcripts comprise two main isoforms: K-Ras 4A and K-Ras 4B, which act differently. The expression of both isoforms was reported in many human tissues. However, K-Ras 4B was the major expressed transcript variant. An increased expression of K-Ras 4B mRNA was reported in eutopic endometrium of endometriosis patients. In this way, we aimed to study the expression of K-Ras 4A transcript in eutopic endometrium related to endometriosis. METHODS: Employing exon4-flanking primers, K-Ras isoforms were simultaneously amplified in a RT-PCR reaction. Quantitative real-time PCR was performed using GAPDH as an internal control. K-Ras 4A transcript expression in eutopic endometrium was analyzed by DeltaDeltaC T method. RESULTS: We identified existence of both of K-Ras 4A and K-Ras 4B in eutopic endometrium of patients and controls. Quantitative real-time analysis demonstrated that K-Ras 4A expression was 2.7-fold higher in endometriosis than non-endometriosis eutopic samples. Interestingly, this overexpression mainly occurs through the proliferative phase of menstrual cycle. CONCLUSION: The findings bring to light the eminent role of K-Ras 4A in endometriosis. This splice variant which is known for promoting apoptosis could be an effective factor in balance between proliferation and death of eutopic endometrial cells. Mesh Terms: Adult| Case-Control Studies| DNA Primers/genetics| Endometriosis/genetics/*pathology| Endometrium/*pathology| Female| Humans| Menstrual Cycle/*physiology| Middle Aged| Proto-Oncogene Proteins/*genetics| Proto-Oncogene Proteins p21(ras)| RNA, Me |
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